Advance online publication
The latest research papers, published online ahead of print. These online versions are definitive and may be cited using the digital object identifier (DOI).
About advance online publicationArticles
Cell stimulation with optically manipulated microsources
Holger Kress, Jin-Gyu Park, Cecile O Mejean, Jason D Forster, Jason Park, Spencer S Walse, Yong Zhang, Dianqing Wu, Orion D Weiner, Tarek M Fahmy & Eric R Dufresne
Published online: 15 November 2009 | doi:10.1038/nmeth.1400
Microsources positioned with holographic optical tweezers can establish a highly localized, three-dimensional chemical gradient that allows the manipulation of polarization and migration in single cells.
Abstract - Cell stimulation with optically manipulated microsources | Full Text - Cell stimulation with optically manipulated microsources | PDF (2,208 KB) - Cell stimulation with optically manipulated microsources | Supplementary information
Transgenic microRNA inhibition with spatiotemporal specificity in intact organisms
Carlos M Loya, Cecilia S Lu, David Van Vactor & Tudor A Fulga
Published online: 15 November 2009 | doi:10.1038/nmeth.1402
Tissue-specific expression of microRNA sponges allows precise regulation of microRNA activity in living flies. The authors investigate the role of miR-8 in the formation of neuromuscular junctions in detail.
Abstract - Transgenic microRNA inhibition with spatiotemporal specificity in intact organisms | Full Text - Transgenic microRNA inhibition with spatiotemporal specificity in intact organisms | PDF (1,640 KB) - Transgenic microRNA inhibition with spatiotemporal specificity in intact organisms | Supplementary information
An auxin-based degron system for the rapid depletion of proteins in nonplant cells
Kohei Nishimura, Tatsuo Fukagawa, Haruhiko Takisawa, Tatsuo Kakimoto & Masato Kanemaki
Published online: 15 November 2009 | doi:10.1038/nmeth.1401
A degradation pathway found in plants, dependent on the hormone auxin, can be transplanted and harnessed to induce rapid and reversible target protein degradation in both yeast and animal cells.
Abstract - An auxin-based degron system for the rapid depletion of proteins in nonplant cells | Full Text - An auxin-based degron system for the rapid depletion of proteins in nonplant cells | PDF (1,069 KB) - An auxin-based degron system for the rapid depletion of proteins in nonplant cells | Supplementary information
A genetically encoded reporter of synaptic activity in vivo
Elena Dreosti, Benjamin Odermatt, Mario M Dorostkar & Leon Lagnado
Published online: 08 November 2009 | doi:10.1038/nmeth.1399
Fusion of the genetically-encoded calcium indicator GCaMP2 to synaptophysin localizes the sensor to neuron presynaptic terminals and conveys linear responsiveness over a wider range of spike frequencies. The sensor allowed measurement of synaptic activity caused by spiking as well as graded voltage signals during in vivo imaging in zebrafish.
Abstract - A genetically encoded reporter of synaptic activity : in vivo | Full Text - A genetically encoded reporter of synaptic activity in vivo | PDF (1,552 KB) - A genetically encoded reporter of synaptic activity in vivo | Supplementary information
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Automated high-throughput mapping of promoter-enhancer interactions in zebrafish embryos
Jochen Gehrig, Markus Reischl, Éva Kalmár, Marco Ferg, Yavor Hadzhiev, Andreas Zaucker, Chengyi Song, Simone Schindler, Urban Liebel & Ferenc Müller
Published online: 08 November 2009 | doi:10.1038/nmeth.1396
Methods for automated fluorescence imaging allow high-throughput examination of reporter expression patterns in zebrafish embryos. They are applied to mapping promoter-enhancer interactions in this organism.
Abstract - Automated high-throughput mapping of promoter-enhancer interactions in zebrafish embryos | Full Text - Automated high-throughput mapping of promoter-enhancer interactions in zebrafish embryos | PDF (1,630 KB) - Automated high-throughput mapping of promoter-enhancer interactions in zebrafish embryos | Supplementary information
Optical interrogation of neural circuits in Caenorhabditis elegans
Zengcai V Guo, Anne C Hart & Sharad Ramanathan
Published online: 08 November 2009 | doi:10.1038/nmeth.1397
Neuronal stimulation with channelrhodopsin-2 is combined with calcium fluorescence imaging to study neural connections in intact Caenorhabditis elegans.
Abstract - Optical interrogation of neural circuits in : Caenorhabditis elegans | Full Text - Optical interrogation of neural circuits in Caenorhabditis elegans | PDF (739 KB) - Optical interrogation of neural circuits in Caenorhabditis elegans | Supplementary information
Imaging neural activity in worms, flies and mice with improved GCaMP calcium indicators
Lin Tian, S Andrew Hires, Tianyi Mao, Daniel Huber, M Eugenia Chiappe, Sreekanth H Chalasani, Leopoldo Petreanu, Jasper Akerboom, Sean A McKinney, Eric R Schreiter, Cornelia I Bargmann, Vivek Jayaraman, Karel Svoboda & Loren L Looger
Published online: 08 November 2009 | doi:10.1038/nmeth.1398
An improved version of the GCaMP genetically encoded calcium indicator, called GCaMP3, has higher calcium affinity and increased baseline fluorescence, dynamic range and stability. GCaMP3 performs better than existing genetically encoded calcium indicators in several assays and organisms, including in vivo imaging of neuronal signaling in worms, flies and mice.
Abstract - Imaging neural activity in worms, flies and mice with improved GCaMP calcium indicators | Full Text - Imaging neural activity in worms, flies and mice with improved GCaMP calcium indicators | PDF (1,235 KB) - Imaging neural activity in worms, flies and mice with improved GCaMP calcium indicators | Supplementary information
High-speed nanoscopic tracking of the position and orientation of a single virus
Philipp Kukura, Helge Ewers, Christian Müller, Alois Renn, Ari Helenius & Vahid Sandoghdar
Published online: 01 November 2009 | doi:10.1038/nmeth.1395
A combination of scattering interferometry and single-molecule fluorescence microscopy allows visualization of both the position and orientation of single Simian virus 40 particles on lipid bilayers and provides evidence of viral interaction with receptors in membrane nanodomains.
Abstract - High-speed nanoscopic tracking of the position and orientation of a single virus | Full Text - High-speed nanoscopic tracking of the position and orientation of a single virus | PDF (618 KB) - High-speed nanoscopic tracking of the position and orientation of a single virus | Supplementary information
Until print versions of AOP papers are published, they should be cited in the style "Author(s) Nature Methods advance online publication, day month year (doi:10.1038/nmethXXXXX)". Once the print version (identical to the AOP) is published, it should be cited as follows: "Author(s) Nature Methods volume, page (year); advance online publication, (doi:10.1038/nmethXXXXX)".
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