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Neonatal desensitization does not universally prevent xenograft rejection

Nature Methods volume 9, pages 856–858 (2012)Cite this article

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The first cell type we tested was immortalized, luciferase-expressing human glial-restricted precursor (hGRP) cells derived from fetal brain (Q Therapeutics). We induced neonatal desensitization by intraperitoneal injection of 1 × 105 hGRP cells into BALB/c mice. The experiment was repeated three times using a total of 37 mice (Supplementary Methods). hGRPs initially produced a strong bioluminescence imaging (BLI) signal; but a dramatic loss of signal was observed within a few days, indicating rapid elimination of transplanted cells in the neonates (Fig. 1a). Once the desensitized animals reached maturity, 3 × 105 hGRP cells were injected into the brain parenchyma; parallel transplantations were conducted in nondesensitized littermates as controls. We monitored the survival of transplanted cells via BLI. Initially, transplanted hGRPs produced a strong BLI signal, which denoted robust survival. However, the signal gradually decreased over time and was undetectable after 2 weeks (Fig. 1b). We observed no difference in the time course of signal loss between desensitized and control animals.

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Figure 1: Xenograft survival in neonatally desensitized animals.

References

  1. Brundin, P. et al. Exp. Brain Res. 70, 192–208 (1988).

    CAS  PubMed  Google Scholar 

  2. Walczak, P. et al. J. Neurosci. Res. 76, 244–254 (2004).

    Article  CAS  PubMed  PubMed Central  Google Scholar 

  3. Liesz, A. et al. Brain 134, 704–720 (2011).

    Article  PubMed  Google Scholar 

  4. Kelly, C.M. Nat. Methods 6, 271–273 (2009).

    Article  CAS  PubMed  Google Scholar 

  5. Shen, Z., Mohiuddin, M., Goldstein, C., Yokoyama, H. & DiSesa, V.J. Transplant. Proc. 28, 719–720 (1996).

    CAS  PubMed  Google Scholar 

  6. Butler, P.E., Lee, W.P., van de Water, A.P. & Randolph, M.A. Plast. Reconstr. Surg. 105, 2424–2430 (2000).

    Article  CAS  PubMed  Google Scholar 

Download references

Acknowledgements

The study was supported by the Maryland Stem Cell Research Fund (MSCRFII0193, MSCRFII0052 and MSCRF06–29–01), the US National Institutes of Health (2RO1 NS045062) and the Polish Ministry of Scientific Research and Higher Education (N401014235). M.J. was supported by a Kolumb Fellowship from the Foundation for Polish Science. The authors thank M. McAllister for editorial assistance.

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  1. Miroslaw Janowski and Anna Jablonska: These authors contributed equally to this work.

Authors and Affiliations

  1. Russell H. Morgan Department of Radiology and Radiological Science, Division of Magnetic Resonance Research, The Johns Hopkins University School of Medicine (JHU SOM), Baltimore, Maryland, USA.,

    Miroslaw Janowski, Inema Orukari, Segun Bernard, Jeff WM Bulte & Piotr Walczak

  2. Cellular Imaging Section and Vascular Biology Program, Institute for Cell Engineering, JHU SOM, Baltimore, Maryland, USA

    Miroslaw Janowski, Inema Orukari, Segun Bernard, Jeff WM Bulte & Piotr Walczak

  3. NeuroRepair Department, Mossakowski Medical Research Centre, Polish Academy of Sciences, Warsaw, Poland

    Miroslaw Janowski, Anna Jablonska, Hanna Kozlowska & Barbara Lukomska

  4. Department of Neurosurgery, Mossakowski Medical Research Centre, Polish Academy of Sciences, Warsaw, Poland

    Miroslaw Janowski

  5. Department of Biomedical Engineering, JHU SOM, Baltimore, Maryland, USA

    Jeff WM Bulte

  6. Department of Chemical & Biomolecular Engineering, JHU SOM, Baltimore, Maryland, USA

    Jeff WM Bulte

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  1. Miroslaw Janowski
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Correspondence to Piotr Walczak.

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Supplementary Figure 1 and Supplementary Methods (PDF 931 kb)

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Janowski, M., Jablonska, A., Kozlowska, H. et al. Neonatal desensitization does not universally prevent xenograft rejection. Nat Methods 9, 856–858 (2012). https://doi.org/10.1038/nmeth.2146

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