Wisedchaisri, G. & Gonen, T. Structure 19, 976–987 (2011).
Electron crystallography is a powerful method for obtaining structural information about membrane proteins from two-dimensional crystals. A challenge, however, is collecting image data that can provide experimental phase information to solve structures at high resolution. Wisedchaisri and Gonen describe a way around this by obtaining phase information from low-resolution images using a fragment-based phase-extension method. This allowed them to solve the high-resolution structures of aquaporin-4, bacteriorhodopsin and aquaporin-0.
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High-resolution structures from low-resolution data. Nat Methods 8, 709 (2011). https://doi.org/10.1038/nmeth.1692
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DOI: https://doi.org/10.1038/nmeth.1692