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Long-term single-cell imaging of mammalian stem cells

Nature Methods volume 8, pages S30–S35 (2011)Cite this article

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Abstract

Continuous long-term single-cell observation provides insight into the molecular control of cell fate. This is particularly important for rare and heterogeneous populations of cells, such as mammalian stem cells. The current lack of usable off-the-shelf hardware and software for such experiments makes their implementation technically challenging. Here I discuss the need for continuous single-cell quantification to understand molecular cell fate control as well as organizational and technical solutions for long-term imaging and tracking of stem cells.

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Figure 1: Continuous single-cell analyses are essential for understanding molecular cell fate control.
Figure 2
Figure 3: Real-time detection of surface markers on living cells with antibodies.

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Acknowledgements

This work was in part financed by the Deutsche Forschungsgemeinschaft.

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  1. Institute of Stem Cell Research, Helmholtz Zentrum Munich–German Research Center for Environmental Health GmbH, Neuherberg, Germany

    Timm Schroeder

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  1. Timm Schroeder
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Correspondence to Timm Schroeder.

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Schroeder, T. Long-term single-cell imaging of mammalian stem cells. Nat Methods 8 (Suppl 4), S30–S35 (2011). https://doi.org/10.1038/nmeth.1577

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