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The cover image artistically depicts the isolation and analysis of a single cell from within a heterogeneous cellular population. Cover design by Erin Dewalt. Supplement Foreword p307
Meeting organizers and speakers are increasingly open to blogging and microblogging—an encouraging trend that should be expanded with clearly defined restrictions.
A wearable imaging device allows functional whole-brain imaging of awake, freely moving rats. This technology opens up a noninvasive window for simultaneously assessing brain function and behavior in response to a wide variety of interventions in living rats.
Confined photoactivation of photoactivatable mCherry using two-photon illumination with line-scanning temporal focusing in combination with three-dimensional localization algorithms allows three-dimensional super-resolution microscopy of cellular features at <50 nm lateral and <100 nm axial resolution and depths greater than 8 μm.
The combination of an ultrahigh-resolution dual optical trap with a confocal microscope allowed single-fluorophore detection of labeled oligonucleotide binding and simultaneous measurement of angstrom-scale changes in DNA tether extension.
RNAi screening combined with automated microscopy of Drosophila melanogaster cells reveal genetic interactions and allow the reconstruction of signaling pathways.
A miniature portable tomograph allows the imaging of awake, behaving rats with positron emission tomography while simultaneously measuring their behavior. This method for noninvasive whole-brain imaging can link studies of brain activity with particular behaviors.
Analyzing the first and higher-order moments of the diffraction spot of a 4Pi fluorescence detection scheme facilitates two-color, three-dimensional super-resolution microscopy with ~6 nm axial and ~8–23 nm lateral resolution in a layer ~650 nm thick.