Kladwang, W. et al. Nat. Chem. advance online publication (30 October 2011).

Kladwang et al. describe a 'mutate-and-map' strategy for inferring RNA structures, involving systematic mutagenesis of one nucleotide in a base pair, combined with high-throughput, single-nucleotide-resolution chemical accessibility mapping. This approach allowed them to model secondary structures of domains from diverse noncoding RNAs, including ribosomal RNA, ribozymes and riboswitches.