The low cost of short-read sequencing has motivated the development of de novo assemblies from only short-read data; impressively, assemblies for large mammalian genomes are now available. However, this is still a developing field, and these de novo assemblies have many artifacts, as do all de novo assemblies.
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Birney, E. Assemblies: the good, the bad, the ugly. Nat Methods 8, 59–60 (2011). https://doi.org/10.1038/nmeth0111-59
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DOI: https://doi.org/10.1038/nmeth0111-59
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