Brief Communication abstract


Nature Methods 7, 303 - 305 (2010)
Published online: 14 March 2010 | doi:10.1038/nmeth.1440

In vivo wide-area cellular imaging by side-view endomicroscopy

Pilhan Kim1, Euiheon Chung2, Hiroshi Yamashita2, Kenneth E Hung3, Atsushi Mizoguchi4, Raju Kucherlapati5, Dai Fukumura2, Rakesh K Jain2 & Seok H Yun1,6,7

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In vivo imaging of small animals offers several possibilities for studying normal and disease biology, but visualizing organs with single-cell resolution is challenging. We describe rotational side-view confocal endomicroscopy, which enables cellular imaging of gastrointestinal and respiratory tracts in mice and may be extensible to imaging organ parenchyma such as cerebral cortex. We monitored cell infiltration, vascular changes and tumor progression during inflammation and tumorigenesis in colon over several months.

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  1. Wellman Center for Photomedicine, Department of Dermatology, Harvard Medical School and Massachusetts General Hospital, Boston, Massachusetts, USA.
  2. Edwin L. Steele Lab for Tumor Biology, Department of Radiation Oncology, Harvard Medical School and Massachusetts General Hospital, Boston, Massachusetts, USA.
  3. Division of Gastroenterology, Tufts Medical Center, Boston, Massachusetts, USA.
  4. Department of Pathology, Center for the Study of Inflammatory Bowel Disease, Harvard Medical School and Massachusetts General Hospital, Boston, Massachusetts, USA.
  5. Department of Genetics, Brigham and Women's Hospital, Boston, Massachusetts, USA.
  6. Graduate School of Nanoscience and Technology, Korea Advanced Institute of Science and Technology, Yuseong-gu, Daejeon, Korea.
  7. The Harvard–Massachusetts Institute of Technology Division of Health Sciences and Technology, Cambridge, Massachusetts, USA.

Correspondence to: Seok H Yun1,6,7 e-mail: syun@hms.harvard.edu



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