A surprisingly simple method provides an effective way of correcting optical distortions in two-photon fluorescence microscopy and recovers nearly ideal images of inhomogeneous thick samples.
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References
Ji, N., Milkie, D.E. & Betzig, E. Nat. Methods 7, 141–147 (2010).
Steinecker, A. & Mader, W. Ultramicroscopy 81, 149–161 (2000).
Ma, M., Wang, X. & Wang, F. Appl. Opt. 45, 8200–8208 (2006).
Levoy, M. et al. ACM Trans. Graphics (Proc. SIGGRAPH) 25, 924–934 (2006).
Levoy, M., Zhang, Z. & McDowall, I. J. Microscopy 235, 144–162 (2009).
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Heintzmann, R. Correcting distorted optics: back to the basics. Nat Methods 7, 108–110 (2010). https://doi.org/10.1038/nmeth0210-108
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DOI: https://doi.org/10.1038/nmeth0210-108