Review abstract

Nature Methods 7, 111 - 118 (2010)
Published online: 28 January 2010 | Corrected online: 12 April 2010 | doi:10.1038/nmeth.1419

There is an Erratum (June 2010) associated with this Review.

Target-enrichment strategies for next-generation sequencing

Lira Mamanova1, Alison J Coffey1, Carol E Scott1, Iwanka Kozarewa1, Emily H Turner2, Akash Kumar2, Eleanor Howard1, Jay Shendure2 & Daniel J Turner1

We have not yet reached a point at which routine sequencing of large numbers of whole eukaryotic genomes is feasible, and so it is often necessary to select genomic regions of interest and to enrich these regions before sequencing. There are several enrichment approaches, each with unique advantages and disadvantages. Here we describe our experiences with the leading target-enrichment technologies, the optimizations that we have performed and typical results that can be obtained using each. We also provide detailed protocols for each technology so that end users can find the best compromise between sensitivity, specificity and uniformity for their particular project.

  1. The Wellcome Trust Sanger Institute, Wellcome Trust Genome Campus, Hinxton, Cambridge, UK.
  2. Department of Genome Sciences, University of Washington, Seattle, Washington, USA.

Correspondence to: Jay Shendure2 e-mail:

Correspondence to: Daniel J Turner1 e-mail:

* In the version of this article initially published, the publication date was incorrectly designated as 28 January 2009 instead of 28 January 2010. The error has been corrected in the HTML and PDF versions of the article.


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