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ErythRED, a hESC line enabling identification of erythroid cells

Nature Methods volume 6, pages 659–662 (2009)Cite this article

Abstract

A human embryonic stem cell (hESC) line that enabled globin-expressing cells to be easily recognized would facilitate optimization of erythroid differentiation in vitro and aid in the identification of hESC-derived erythroid cells in transplanted animals. We describe a genetically modified hESC line, ErythRED, in which expression of RFP, controlled by regulatory sequences from the human β-globin locus control region, is restricted to maturing erythroid cells.

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Figure 1: Red fluorescence is localized to hemoglobinized erythroid cells differentiated from ErythRED hESCs.
Figure 2: RFP expression identifies erythroid cells in vitro.
Figure 3: ErythRED embyoid bodies generate RFP+ erythroid cells in vivo.

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Acknowledgements

This work was supported by research grants from the Australian Stem Cell Centre, the Juvenile Diabetes Research Foundation and the National Health and Medical Research Council of Australia.

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Author notes

  1. Edouard G Stanley and Andrew G Elefanty: These authors contributed equally to this work.

Authors and Affiliations

  1. Monash Immunology and Stem Cell Laboratories, Monash University, Clayton, Victoria, Australia

    Tanya Hatzistavrou, Suzanne J Micallef, Elizabeth S Ng, Edouard G Stanley & Andrew G Elefanty

  2. Cell and Gene Therapy Group, Murdoch Childrens Research Institute, Royal Children's Hospital, Parkville, Victoria, Australia

    Jim Vadolas

Authors
  1. Tanya Hatzistavrou
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  2. Suzanne J Micallef
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  3. Elizabeth S Ng
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  5. Edouard G Stanley
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  6. Andrew G Elefanty
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Contributions

T.H. generated the ErythRED cell line, designed and performed differentiation experiments, flow cytometry and wrote the paper; S.J.M. performed transplantation experiments; E.S.N. designed and performed differentiation experiments and intracellular flow cytometry; J.V. performed HPLC and mass spectrometry experiments; E.G.S. designed experiments, wrote the paper and had final approval of the manuscript; A.G.E. designed and performed experiments, wrote the paper and had final approval of the manuscript.

Corresponding author

Correspondence to Andrew G Elefanty.

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Supplementary Figures 1–5 and Supplementary Table 1 (PDF 640 kb)

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Hatzistavrou, T., Micallef, S., Ng, E. et al. ErythRED, a hESC line enabling identification of erythroid cells. Nat Methods 6, 659–662 (2009). https://doi.org/10.1038/nmeth.1364

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