Brief Communication abstract

Nature Methods 6, 593 - 595 (2009)
Published online: 20 July 2009 | doi:10.1038/nmeth.1354

Virtual terminator nucleotides for next-generation DNA sequencing

Jayson Bowers1, Judith Mitchell1, Eric Beer1, Philip R Buzby1, Marie Causey1, J William Efcavitch1, Mirna Jarosz1, Edyta Krzymanska-Olejnik1, Li Kung1, Doron Lipson1, Geoffrey M Lowman1, Subramanian Marappan1, Peter McInerney1, Adam Platt1, Atanu Roy1, Suhaib M Siddiqi1, Kathleen Steinmann1 & John F Thompson1


We synthesized reversible terminators with tethered inhibitors for next-generation sequencing. These were efficiently incorporated with high fidelity while preventing incorporation of additional nucleotides, and we used them to sequence canine bacterial artificial chromosomes in a single-molecule system that provided even coverage for over 99% of the region sequenced. This single-molecule approach generated high-quality sequence data without the need for target amplification and thus avoided concomitant biases.

  1. Helicos BioSciences Corporation, Cambridge, Massachusetts, USA.

Correspondence to: John F Thompson1 e-mail:


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