Brief Communication abstract


Nature Methods 6, 581 - 583 (2009)
Published online: 13 July 2009 | doi:10.1038/nmeth.1352

Global discovery of adaptive mutations

Hani Goodarzi1,2, Alison K Hottes1,2 & Saeed Tavazoie1

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Although modern DNA sequencing enables rapid identification of genetic variation, characterizing the phenotypic consequences of individual mutations remains a labor-intensive task. Here we describe array-based discovery of adaptive mutations (ADAM), a technology that searches an entire bacterial genome for mutations that contribute to selectable phenotypic variation between an evolved strain and its parent. We found that ADAM identified adaptive mutations in laboratory-evolved Escherichia coli strains with high sensitivity and specificity.

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  1. Department of Molecular Biology & Lewis-Sigler Institute for Integrative Genomics, Princeton University, Princeton, New Jersey, USA.
  2. These authors contributed equally to this work.

Correspondence to: Saeed Tavazoie1 e-mail: tavazoie@genomics.princeton.edu



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