Brief Communication abstract
Nature Methods 6, 585 - 587 (2009)
Published online: 5 July 2009 | doi:10.1038/nmeth.1347
Mass spectrometry of membrane transporters reveals subunit stoichiometry and interactions
Nelson P Barrera1, Shoshanna C Isaacson1, Min Zhou1, Vassiliy N Bavro2, Alex Welch3, Theresia A Schaedler3, Markus A Seeger3, Ricardo Núñez Miguel4, Vladimir M Korkhov5, Hendrik W van Veen3, Henrietta Venter3, Adrian R Walmsley6, Christopher G Tate5 & Carol V Robinson1
We describe a general mass spectrometry approach to determine subunit stoichiometry and lipid binding in intact membrane protein complexes. By exploring conditions for preserving interactions during transmission into the gas phase and for optimally stripping away detergent, by subjecting the complex to multiple collisions, we released the intact complex largely devoid of detergent. This enabled us to characterize both subunit stoichiometry and lipid binding in 4 membrane protein complexes.
- Department of Chemistry, University of Cambridge, Cambridge, UK.
- Department of Physics, University of Oxford, Clarendon Laboratory, Oxford, UK.
- Department of Pharmacology, University of Cambridge, Cambridge, UK.
- Department of Biochemistry, University of Cambridge, Cambridge, UK.
- Medical Research Council Laboratory of Molecular Biology, Cambridge, UK.
- School of Biological and Biomedical Sciences, Durham University, Durham, UK.
Correspondence to: Carol V Robinson1 e-mail: cvr24@cam.ac.uk
MORE ARTICLES LIKE THIS
These links to content published by NPG are automatically generated.
RESEARCH
Dissociation of the dimeric SecA ATPase during protein translocation across the bacterial membraneThe EMBO Journal Article (02 Sep 2002)
Binding, activation and dissociation of the dimeric SecA ATPase at the dimeric SecYEG translocaseThe EMBO Journal Article (01 Sep 2003)
Binding, activation and dissociation of the dimeric SecA ATPase at the dimeric SecYEG translocaseThe EMBO Journal Article (01 Sep 2003)
Parallel topology of genetically fused EmrE homodimersThe EMBO Journal Article (09 Jan 2008)
