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Brief Communication

Nature Methods 6, 493–495 (1 July 2009) | doi:10.1038/nmeth.1342

Agouti C57BL/6N embryonic stem cells for mouse genetic resources

Stephen J Pettitt , Qi Liang , Xin Y Rairdan , Jennifer L Moran , Haydn M Prosser , David R Beier , Kent C Lloyd , Allan Bradley & William C Skarnes

We report the characterization of a highly germline competent C57BL/6N mouse embryonic stem cell line, JM8. To simplify breeding schemes, the dominant agouti coat color gene was restored in JM8 cells by targeted repair of the C57BL/6 nonagouti mutation.