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Nature Methods 6, 515–519 (1 July 2009) | doi:10.1038/nmeth.1336

Reaching the protein folding speed limit with large, sub-microsecond pressure jumps

Charles Dumont , Tryggvi Emilsson & Martin Gruebele

Biomolecules are highly pressure-sensitive, but their dynamics upon return to ambient pressure are often too fast to observe with existing approaches. We describe a sample-efficient method capable of large and very fast pressure drops (2,500 atmospheres and