Brief Communication abstract
Nature Methods 6, 497 - 499 (2009)
Published online: 21 June 2009 | doi:10.1038/nmeth.1344
Simultaneous detection of many T-cell specificities using combinatorial tetramer staining
Evan W Newell1, Lawrence O Klein2, Wong Yu3 & Mark M Davis1,2,4
The direct detection of antigen-specific T cells using tetramers of soluble peptide–major histocompatibilty complex (pMHC) molecules is widely used in both basic and clinical immunology. However, the number of specificities that can be assessed simultaneously has been a major limitation. Here we describe and validate a method using combinations of fluorescent pMHC tetramers to simultaneously detect and enrich for many (
15) T-cell specificities in a single human blood sample.
- Department of Microbiology and Immunology, Stanford University School of Medicine, Stanford, California, USA.
- Program in Biophysics, Stanford University School of Medicine, Stanford, California, USA.
- Division of Hematology, Stanford University School of Medicine, Stanford, California, USA.
- The Howard Hughes Medical Institute, Stanford University School of Medicine, Stanford, California, USA.
Correspondence to: Mark M Davis1,2,4 e-mail: mmdavis@stanford.edu
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