Brief Communication abstract
Nature Methods 6, 493 - 495 (2009)
Published online: 14 June 2009 | doi:10.1038/nmeth.1342
Agouti C57BL/6N embryonic stem cells for mouse genetic resources
Stephen J Pettitt1, Qi Liang1, Xin Y Rairdan2, Jennifer L Moran3,4, Haydn M Prosser1, David R Beier3, Kent C Lloyd2,4, Allan Bradley1 & William C Skarnes1
We report the characterization of a highly germline competent C57BL/6N mouse embryonic stem cell line, JM8. To simplify breeding schemes, the dominant agouti coat color gene was restored in JM8 cells by targeted repair of the C57BL/6 nonagouti mutation. These cells provide a robust foundation for large-scale mouse knockout programs that aim to provide a public resource of targeted mutations in the C57BL/6 genetic background.
- Wellcome Trust Sanger Institute, Wellcome Trust Genome Campus, Cambridge, UK.
- Center for Comparative Medicine, School of Veterinary Medicine, University of California, Davis, California, USA.
- Brigham and Women's Hospital, Genetics Division, Harvard Medical School, Boston, Massachusetts, USA.
- Present addresses: Broad Institute of Massachusetts Institute of Technology and Harvard, Cambridge, Massachusetts, USA (J.L.M.) and Stanley Center for Psychiatric Research, Broad Institute of Massachusetts Institute of Technology and Harvard, Cambridge, Massachusetts, USA (K.C.L.).
Correspondence to: William C Skarnes1 e-mail: skarnes@sanger.ac.uk
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