Brief Communication abstract
Nature Methods 6, 511 - 512 (2009)
Published online: 14 June 2009 | doi:10.1038/nmeth.1339
In vivo fluorescence imaging with high-resolution microlenses
Robert P J Barretto1, Bernhard Messerschmidt2 & Mark J Schnitzer1,3
Micro-optics are increasingly used for minimally invasive in vivo imaging, in miniaturized microscopes and in lab-on-a-chip devices. Owing to optical aberrations and lower numerical apertures, a main class of microlens, gradient refractive index lenses, has not achieved resolution comparable to conventional microscopy. Here we describe high-resolution microlenses, and illustrate two-photon imaging of dendritic spines on hippocampal neurons and dual-color nonlinear optical imaging of neuromuscular junctions in live mice.
- James H. Clark Center for Biomedical Engineering and Sciences, Stanford University, Stanford, California, USA.
- GRINTECH GmbH, Jena, Germany.
- Howard Hughes Medical Institute, Stanford University, Stanford, California, USA.
Correspondence to: Mark J Schnitzer1,3 e-mail: mschnitz@stanford.edu
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