Brief Communication abstract
Nature Methods 6, 503 - 506 (2009)
Published online: 14 June 2009 | doi:10.1038/nmeth.1338
Quantitative analysis of gene expression in a single cell by qPCR
Kiyomi Taniguchi1, Tomoharu Kajiyama1 & Hideki Kambara1
We developed a quantitative PCR method featuring a reusable single-cell cDNA library immobilized on beads for measuring the expression of multiple genes in a single cell. We used this method to analyze multiple cDNA targets (from several copies to several hundred thousand copies) with an experimental error of 15.9% or less. This method is sufficiently accurate to investigate the heterogeneity of single cells.
- Hitachi Central Research Laboratory, Tokyo, Japan.
Correspondence to: Hideki Kambara1 e-mail: hideki.kambara.se@hitachi.com
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