Brief Communication abstract
Nature Methods 6, 431 - 434 (2009)
Published online: 24 May 2009 | doi:10.1038/nmeth.1331
Versatile P[acman] BAC libraries for transgenesis studies in Drosophila melanogaster
Koen J T Venken1, Joseph W Carlson2,8, Karen L Schulze3,8, Hongling Pan3, Yuchun He3, Rebecca Spokony4, Kenneth H Wan2, Maxim Koriabine5, Pieter J de Jong5, Kevin P White4, Hugo J Bellen1,3,6,7 & Roger A Hoskins2
We constructed Drosophila melanogaster bacterial artificial chromosome libraries with 21-kilobase and 83-kilobase inserts in the P[acman] system. We mapped clones representing 12-fold coverage and encompassing more than 95% of annotated genes onto the reference genome. These clones can be integrated into predetermined attP sites in the genome using 
C31 integrase to rescue mutations. They can be modified through recombineering, for example, to incorporate protein tags and assess expression patterns.
- Department of Molecular and Human Genetics, Baylor College of Medicine, Houston, Texas, USA.
- Life Sciences Division, Lawrence Berkeley National Laboratory, Berkeley, California, USA.
- Howard Hughes Medical Institute, Baylor College of Medicine, Houston, Texas, USA.
- Institute for Genomics and Systems Biology and Department of Human Genetics, University of Chicago, Chicago, Illinois, USA.
- Children's Hospital Oakland Research Institute, Oakland, California, USA.
- Department of Neuroscience, Baylor College of Medicine, Houston, Texas, USA.
- Program in Developmental Biology, Baylor College of Medicine, Houston, Texas, USA.
- These authors contributed equally to this work.
Correspondence to: Hugo J Bellen1,3,6,7 e-mail: hbellen@bcm.tmc.edu
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