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Brief Communication

Nature Methods 6, 355–358 (1 May 2009) | doi:10.1038/nmeth.1319

Photoconversion in orange and red fluorescent proteins

Gert-Jan Kremers , Kristin L Hazelwood , Christopher S Murphy , Michael W Davidson & David W Piston

We found that photoconversion is fairly common among orange and red fluorescent proteins, as in a screen of 12 proteins, 8 exhibited photoconversion. Specifically, three red fluorescent proteins could be switched to a green state, and two orange variants could be photoconverted to a far-red state.