Brief Communication abstract
Nature Methods 6, 359 - 362 (2009)
Published online: 19 April 2009 | doi:10.1038/nmeth.1322
Universal sample preparation method for proteome analysis
Jacek R Wi
niewski1,
Alexandre Zougman1,
Nagarjuna Nagaraj1
&
Matthias Mann1
We describe a method, filter-aided sample preparation (FASP), which combines the advantages of in-gel and in-solution digestion for mass spectrometry–based proteomics. We completely solubilized the proteome in sodium dodecyl sulfate, which we then exchanged by urea on a standard filtration device. Peptides eluted after digestion on the filter were pure, allowing single-run analyses of organelles and an unprecedented depth of proteome coverage.
- Department of Proteomics and Signal Transduction, Max Planck Institute for Biochemistry, Martinsried, Germany.
Correspondence to: Jacek R Winiewski1
e-mail: jwisniew@biochem.mpg.de
Correspondence to: Matthias Mann1 e-mail: mmann@biochem.mpg.de
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