Brief Communication abstract


Nature Methods 6, 351 - 353 (2009)
Published online: 6 April 2009 | doi:10.1038/nmeth.1317

An ultramarine fluorescent protein with increased photostability and pH insensitivity

Wataru Tomosugi1, Tomoki Matsuda1, Tomomi Tani1, Tomomi Nemoto2, Ippei Kotera1, Kenta Saito3, Kazuki Horikawa3 & Takeharu Nagai1,3

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We report a pH-insensitive and photostable ultramarine fluorescent protein, Sirius, with an emission peak at 424 nm, the shortest emission wavelength among fluorescent proteins reported to date. The pH-insensitivity of Sirius allowed prolonged visualization of biological events in an acidic environment. Two fluorescence resonance energy transfer (FRET) pairs, Sirius-mseCFP and Sapphire-DsRed, allowed dual-FRET imaging with single-wavelength excitation, enabling detection of Ca2+ concentration and caspase-3 activation in the same apoptotic cells.

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  1. Laboratory for Nanosystems Physiology, Research Institute for Electronic Science, Hokkaido University, Sapporo, Hokkaido, Japan.
  2. Supportive Center for Brain Research, National Institute for Physiological Science, Okazaki, Aichi, Japan.
  3. Nikon Imaging Center, Research Institute for Electronic Science, Hokkaido University, Sapporo, Hokkaido, Japan.

Correspondence to: Takeharu Nagai1,3 e-mail: tnagai@es.hokudai.ac.jp



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