Brief Communication abstract
Nature Methods 6, 351 - 353 (2009)
Published online: 6 April 2009 | doi:10.1038/nmeth.1317
An ultramarine fluorescent protein with increased photostability and pH insensitivity
Wataru Tomosugi1, Tomoki Matsuda1, Tomomi Tani1, Tomomi Nemoto2, Ippei Kotera1, Kenta Saito3, Kazuki Horikawa3 & Takeharu Nagai1,3
We report a pH-insensitive and photostable ultramarine fluorescent protein, Sirius, with an emission peak at 424 nm, the shortest emission wavelength among fluorescent proteins reported to date. The pH-insensitivity of Sirius allowed prolonged visualization of biological events in an acidic environment. Two fluorescence resonance energy transfer (FRET) pairs, Sirius-mseCFP and Sapphire-DsRed, allowed dual-FRET imaging with single-wavelength excitation, enabling detection of Ca2+ concentration and caspase-3 activation in the same apoptotic cells.
- Laboratory for Nanosystems Physiology, Research Institute for Electronic Science, Hokkaido University, Sapporo, Hokkaido, Japan.
- Supportive Center for Brain Research, National Institute for Physiological Science, Okazaki, Aichi, Japan.
- Nikon Imaging Center, Research Institute for Electronic Science, Hokkaido University, Sapporo, Hokkaido, Japan.
Correspondence to: Takeharu Nagai1,3 e-mail: tnagai@es.hokudai.ac.jp
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