Brief Communication abstract
Nature Methods 6, 347 - 349 (2009)
Published online: 6 April 2009 | doi:10.1038/nmeth.1316
Single molecule–sensitive probes for imaging RNA in live cells
Philip J Santangelo1, Aaron W Lifland1, Paul Curt1, Yukio Sasaki2, Gary J Bassell2, Michael E Lindquist3 & James E Crowe Jr3,4
To visualize native or non-engineered RNA in live cells with single-molecule sensitivity, we developed multiply labeled tetravalent RNA imaging probes (MTRIPs). When delivered with streptolysin O into living human epithelial cancer cells and primary chicken fibroblasts, MTRIPs allowed the accurate imaging of native mRNAs and a non-engineered viral RNA, of RNA co-localization with known RNA-binding proteins, and of RNA dynamics and interactions with stress granules.
- Wallace H. Coulter Department of Biomedical Engineering, Georgia Institute of Technology and Emory University, Atlanta, Georgia, USA.
- Department of Cell Biology, Emory University School of Medicine, Atlanta, Georgia, USA.
- Department of Microbiology and Immunology, Vanderbilt University School of Medicine, Nashville, Tennessee, USA.
- Department of Pediatrics, Vanderbilt University School of Medicine, Nashville, Tennessee, USA.
Correspondence to: Philip J Santangelo1 e-mail: philip.santangelo@bme.gatech.edu
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