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Brief Communication

Nature Methods 6, 131–133 (1 February 2009) | doi:10.1038/nmeth.1296

A bright and photostable photoconvertible fluorescent protein

Sean A McKinney , Christopher S Murphy , Kristin L Hazelwood , Michael W Davidson & Loren L Looger

Photoconvertible fluorescent proteins are potential tools for investigating dynamic processes in living cells and for emerging super-resolution microscopy techniques. Unfortunately, most probes in this class are hampered by oligomerization, small photon budgets or poor photostability.