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Article
Nature Methods 6, 161–166 (1 February 2009) | doi:10.1038/nmeth.1288
A genetically encoded fluorescent reporter of ATP:ADP ratio
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Abstract
We constructed a fluorescent sensor of adenylate nucleotides by combining a circularly permuted variant of GFP with a bacterial regulatory protein, GlnK1, from Methanococcus jannaschii. The sensor's affinity for Mg-ATP was
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