Brief Communication abstract
Nature Methods 6, 127 - 130 (2009)
Published online: 4 January 2009 | doi:10.1038/nmeth.1292
Genetically timed, activity-sensor and rainbow transsynaptic viral tools
Zsolt Boldogk
i1,3,
Kamill Balint2,3,
Gautam B Awatramani2,
David Balya2,
Volker Busskamp2,
Tim James Viney2,
Pamela S Lagali2,
Jens Duebel2,
Emese Pásti1,
Dóra Tombácz1,
Judit S Tóth1,
Irma F Takács1,
Brigitte Gross Scherf2
&
Botond Roska2
We developed retrograde, transsynaptic pseudorabies viruses (PRVs) with genetically encoded activity sensors that optically report the activity of connected neurons among spatially intermingled neurons in the brain. Next we engineered PRVs to express two differentially colored fluorescent proteins in a time-shifted manner to define a time period early after infection to investigate neural activity. Finally we used multiple-colored PRVs to differentiate and dissect the complex architecture of brain regions.
- Department of Medical Biology, Faculty of Medicine, University of Szeged, Somogyi B. u. 4., Szeged, H-6720, Hungary.
- Neural Circuit Laboratories, Friedrich Miescher Institute for Biomedical Research, Maulbeerstrasse 66, CH-4058 Basel, Switzerland.
- These authors contributed equally to this work.
Correspondence to: Botond Roska2 e-mail: botond.roska@fmi.ch
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