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Lighting up neural networks using a new generation of genetically encoded calcium sensors

Nature Methods volume 6, pages 871–872 (2009)Cite this article

Abstract

Two improved genetically encoded calcium indicators—based on structure-guided sensor design or on precise subcellular targeting to presynaptic boutons—allow single spikes to be detected in genetically defined populations of neurons and synapses in vivo.

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Figure 1: Strategies used in the development of new GECI variants for imaging activation of neurons and synapses by single spikes in vivo.

References

  1. Tian, L., et al. Nat. Methods 6, 875–881 (2009).

    Article  CAS  Google Scholar 

  2. Dreosti, E., Odermatt, B., Dorostkar, M.M. & Lagnado, L. Nat. Methods 6, 883–889 (2009).

    Article  CAS  Google Scholar 

  3. Scanziani, M. & Häusser, M. Nature 461, 930–939 (2009).

    Article  CAS  Google Scholar 

  4. Stosiek, C., Garaschuk, O., Holthoff, K. & Konnerth, A. Proc. Natl. Acad. Sci. USA 100, 7319–7324 (2003).

    Article  CAS  Google Scholar 

  5. Nagayama, S. et al. Neuron 53, 789–803 (2007).

    Article  CAS  Google Scholar 

  6. Svoboda, K., Denk, W., Kleinfeld, D. & Tank, D.W. Nature 385, 161–165 (1997).

    Article  CAS  Google Scholar 

  7. Mank, M. & Griesbeck, O. Chem. Rev. 108, 1550–1564 (2008).

    Article  CAS  Google Scholar 

  8. Mank, M. et al. Nat. Methods 5, 805–811 (2008).

    Article  CAS  Google Scholar 

  9. Wallace, D.J. et al. Nat. Methods 5, 797–804 (2008).

    Article  CAS  Google Scholar 

  10. Guo, Z.V., Hart, A.C. & Ramanathan, S. Nat. Methods 6, 891–896 (2009).

    Article  CAS  Google Scholar 

  11. Hendel, T. et al. J. Neurosci. 28, 7399–7411 (2008).

    Article  CAS  Google Scholar 

  12. Akerboom, J. et al. J. Biol. Chem. 284, 6455–6464 (2009).

    Article  CAS  Google Scholar 

  13. Miesenböck, G., De Angelis, D.A. & Rothman, J.E. Nature 394, 192–195 (1998).

    Article  Google Scholar 

Download references

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Authors and Affiliations

  1. Christian D. Wilms and Michael Häusser are at the Wolfson Institute for Biomedical Research and Department of Neuroscience, Physiology and Pharmacology, University College London, London, UK.,

    Christian D Wilms & Michael Häusser

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  1. Christian D Wilms
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  2. Michael Häusser
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Correspondence to Michael Häusser.

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Wilms, C., Häusser, M. Lighting up neural networks using a new generation of genetically encoded calcium sensors. Nat Methods 6, 871–872 (2009). https://doi.org/10.1038/nmeth1209-871

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