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Brief Communication
Nature Methods 6, 809–811 (1 November 2009) | doi:10.1038/nmeth.1392
Enrichment of glycopeptides for glycan structure and attachment site identification
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Abstract
We present a method to enrich for glycoproteins from proteomic samples. Sialylated glycoproteins were selectively periodate-oxidized, captured on hydrazide beads, trypsinized and released by acid hydrolysis of sialic acid glycosidic bonds.
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