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Engineered fluorescent proteins: innovations and applications

Despite expansion of the fluorescent protein and optical highlighter palette into the orange to far-red range of the visible spectrum, achieving performance equivalent to that of EGFP has continued to elude protein engineers.

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Figure 1: The fluorescent protein color palette has expanded 140 nm into longer wavelengths over the past 15 years to include many new variants in the orange, red and far-red spectral regions.

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Correspondence to Robert E Campbell.

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R.E.C. is listed as an inventor on US patent applications which include as claims some of the fluorescent proteins described in this manuscript.

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Davidson, M., Campbell, R. Engineered fluorescent proteins: innovations and applications. Nat Methods 6, 713–717 (2009). https://doi.org/10.1038/nmeth1009-713

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