Brief Communication abstract

Nature Methods 6, 71 - 74 (2009)
Published online: 21 December 2008 | doi:10.1038/nmeth.1289

An in vitro microfluidic approach to generating protein-interaction networks

Doron Gerber1, Sebastian J Maerkl1,2 & Stephen R Quake1


We developed an in vitro protein expression and interaction analysis platform based on a highly parallel and sensitive microfluidic affinity assay, and used it for 14,792 on-chip experiments, which exhaustively measured the protein-protein interactions of 43 Streptococcus pneumoniae proteins in quadruplicate. The resulting network of 157 interactions was denser than expected based on known networks. Analysis of the network revealed previously undescribed physical interactions among members of some biochemical pathways.

  1. Department of Bioengineering, Stanford University and Howard Hughes Medical Institute, 318 Campus Drive, Clark Center E300, Stanford, California 94305, USA.
  2. Present address: École Polytechnique Fédérale de Lausanne, Institute of Bioengineering, Station 17, Lausanne, CH-1015, Switzerland.

Correspondence to: Stephen R Quake1 e-mail:


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