Brief Communication abstract


Nature Methods 6, 79 - 81 (2009)
Published online: 14 December 2008 | doi:10.1038/nmeth.1278

Infrared laser–mediated gene induction in targeted single cells in vivo

Yasuhiro Kamei1,2,8, Motoshi Suzuki3,8, Kenjiro Watanabe4,8, Kazuhiro Fujimori2, Takashi Kawasaki2, Tomonori Deguchi2, Yoshihiro Yoneda5, Takeshi Todo1,6, Shin Takagi3, Takashi Funatsu4,7 & Shunsuke Yuba2

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We developed infrared laser–evoked gene operator (IR-LEGO), a microscope system optimized for heating cells without photochemical damage. Infrared irradiation causes reproducible temperature shifts of the in vitro microenvironment in a power-dependent manner. When applied to living Caenorhabditis elegans, IR-LEGO induced heat shock–mediated expression of transgenes in targeted single cells in a more efficient and less deleterious manner than a 440-nm dye laser and elicited physiologically relevant phenotypic responses.

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  1. Radiation Biology Center, Kyoto University, Yoshida Konoe-cho, Sakyo-ku, Kyoto 606-8501, Japan.
  2. National Institute of Advanced and Industrial Science and Technology (AIST), 1-8-31 Midorigaoka, Ikeda, Osaka 563-8577, Japan.
  3. Division of Biological Science, Graduate School of Science, Nagoya University, Furo-cho, Chikusa-ku, Nagoya 464-8602, Japan.
  4. Major in Bioscience and Biomedical Engineering, Waseda University, 3-4-1 Okubo, Shinjuku, Tokyo 169-8855, Japan.
  5. Department of Cell Biology and Neuroscience, Graduate School of Medicine, Osaka University, 2-2 Yamada-oka, Suita, Osaka 565-0871, Japan.
  6. Department of Radiation Biology and Medical Genetics, Graduate School of Medicine, Osaka University, 2-2 Yamada-oka, Suita, Osaka 565-0871, Japan.
  7. Laboratory of Bio-Analytical Chemistry, Graduate School of Pharmaceutical Sciences, The University of Tokyo, 7-3-1 Hongo, Bunkyo, Tokyo 113-0033, Japan.
  8. These authors contributed equally to this work.

Correspondence to: Yasuhiro Kamei1,2,8 e-mail: ykamei@radbio.med.osaka-u.ac.jp

Correspondence to: Shunsuke Yuba2 e-mail: s-yuba@aist.go.jp



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