Application Note abstract
Nature Methods 5, (2008)
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Transcriptome sequencing with the Genome Sequencer FLX system
Abstract
Transcriptome sequencing is a term that encompasses experiments including mRNA transcript-expression analysis (full-length mRNA, expressed sequence tags (ESTs) and ditags), novel gene discovery, gene space identification in novel genomes, assembly of full-length genes, single-nucleotide polymorphism (SNP), insertion-deletion and splice-variant discovery, as well as analyses of allele-specific expression and chromosomal rearrangement. The combination of long, accurate reads and high throughput makes 454 Sequencing™ analysis on the Genome Sequencer FLX ideally suited to detailed transcriptome investigation.
Authors
Thomas Jarvie & Timothy Harkins
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