Nature Methods
- 5, 719 - 725 (2008)
Published online: 11 July 2008; | doi:10.1038/nmeth.1231
Yeast Barcoders: a chemogenomic application of a universal donor-strain collection carrying bar-code identifiersZhun Yan1, 2, Michael Costanzo1, 3, 4, Lawrence E Heisler1, 3, Jadine Paw5, Fiona Kaper6, Brenda J Andrews1, 3, 4, Charles Boone1, 3, 4, Guri Giaever1, 2, 3 & Corey Nislow1, 3, 41
Terrence Donnelly Center for Cellular and Biomolecular Research, University of Toronto, 160 College Street, Toronto, Ontario M5S 3E1, Canada. 2
Department of Pharmaceutical Sciences, University of Toronto, 144 College Street, Toronto, Ontario M5S 3M2, Canada. 3
Department of Molecular Genetics, University of Toronto, King's College Circle, Ontario M5S 1A8, Canada. 4
Banting and Best Department of Medical Research, University of Toronto, 112 College Street, Toronto, Ontario M5G 1L6, Canada. 5
University of Calgary, School of Medicine, 2500 University Dr. NW, Calgary, Alberta T2N 1N4, Canada. 6
Prognosys Biosciences, Inc., 505 Coast Blvd. S., Ste. 302, San Diego, California 92037, USA.
Correspondence should be addressed to Corey Nislow corey.nislow@utoronto.ca The ability to perform complex bioassays in parallel enables experiments that are otherwise impossible because of throughput and cost constraints. For example, highly parallel chemical-genetic screens using pooled collections of thousands of defined Saccharomyces cerevisiae gene deletion strains are feasible because each strain is bar-coded with unique DNA sequences. It is, however, time-consuming and expensive to individually bar-code individual strains. To provide a simple and general method of barcoding yeast collections, we built a set of donor strains, called Barcoders, with unique bar codes that can be systematically transferred to any S. cerevisiae collection. We applied this technology by generating a collection of bar-coded 'decreased abundance by mRNA perturbation' (DAmP) loss-of-function strains comprising 87.1% of all essential yeast genes. These experiments validate both the Barcoders and the DAmP strain collection as useful tools for genome-wide chemical-genetic assays.
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