Nature Methods
- 5, 703 - 709 (2008)
Published online: 29 June 2008; | doi:10.1038/nmeth.1228
Automated analysis of embryonic gene expression with cellular resolution in C. elegansJohn Isaac Murray1, 3, Zhirong Bao1, 2, 3, Thomas J Boyle1, Max E Boeck1, Barbara L Mericle1, Thomas J Nicholas1, Zhongying Zhao1, Matthew J Sandel1 & Robert H Waterston11
Department of Genome Sciences, University of Washington School of Medicine, 1705 NE Pacific Street, Seattle, Washington 98195, USA. 2
Present address: Developmental Biology Program, Sloan-Kettering Institute, 1275 York Avenue, New York, New York 10065, USA. 3
These authors contributed equally to this work.
Correspondence should be addressed to Robert H Waterston waterston@gs.washington.edu We describe a system that permits the automated analysis of reporter gene expression in Caenorhabditis elegans with cellular resolution continuously during embryogenesis. We demonstrate its utility by defining the expression patterns of reporters for several embryonically expressed transcription factors. The invariant cell lineage permits the automated alignment of multiple expression profiles, allowing direct comparison of the expression of different genes' reporters. We also used this system to monitor perturbations to normal development involving changes both in cell-division timing and in cell fate. Systematic application of this system could reveal the gene activity of each cell throughout development.
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