Automated on-chip rapid microscopy, phenotyping and sorting of C. elegans

Kwanghun Chung, Matthew M Crane & Hang Lu

Supplementary Text and Figures (pdf 1M)
Supplementary Figures 1-7, Supplementary Methods

Supplementary Video 1 (mov 2M)
No cooling. This movie shows a nematode being imaged at high magnification (100X) without cooling. A pressure gradient is applied to the worm to push it against the side channels in an attempt to keep the animal from moving. As can be easily seen, the worm is still able to move and rotate significantly.

Supplementary Video 2 (mov 2M)
Cooling. This movie shows a nematode being imaged at high magnification (100X) with cooling applied. Unlike Video 1, no pressure gradient is applied to the animal to keep it in place. With cooling alone the animal remains perfectly still for the entire duration of the imaging process.

Supplementary Video 3 (mov 2M)
Routing. This movie shows worms entering the observation chamber and then being routed to either the top or bottom outlet. This demonstrates the routing process of worms during sorting.

Supplementary Video 4 (mov 1M)
Loading regulator. This movie shows the functioning loading regulator. When a worm is positioned inside the observation chamber, the pressure drop across the worm positioned behind the loading regulator is too small to push it into the imaging area.

Supplementary Video 5 (mov 500K)
AWC 1 ON. Representative z-stack of strain CX3695 at 100x on-chip immobilized using cooling showing only one AWC cell body and dendritic/axonal processes.

Supplementary Video 6 (mov 740K)
AWC 2 ON. Representative z-stack of strain CX3940 at 100x on-chip immobilized using cooling showing two AWC cell bodies and dendritic/axonal processes.

Supplementary Video 7 (mov 652K)
CZ5261. Representative z-stack of strain CZ5261 at 100x on-chip immobilized using cooling.

Supplementary Video 8 (mov 2M)
CZ5264. Representative z-stack of strain CZ5264 at 100x on-chip immobilized using cooling.

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