Nature Methods
- 5, 605 - 607 (2008)
Published online: 8 June 2008; | doi:10.1038/nmeth.1220
Lifeact: a versatile marker to visualize F-actinJulia Riedl, Alvaro H Crevenna, Kai Kessenbrock, Jerry Haochen Yu, Dorothee Neukirchen, Michal Bista, Frank Bradke, Dieter Jenne, Tad A Holak, Zena Werb, Michael Sixt & Roland Wedlich-Soldner Supplementary Text and Figures (pdf 196K)
Supplementary Figure 1, Supplementary Methods Supplementary Movie 1 (mov 2M)
Actin dynamics in a mouse embryonic fibroblast. Images were acquired at 9 s/frame and are played back at 10 frames/s. Time stamp in minutes and seconds. Supplementary Movie 2 (mov 3M)
Actin dynamics in a chemotactic mouse dendritic cell. Images were acquired at 3 s/frame and are played back at 10 frames/s. Time stamp in minutes and seconds. Supplementary Movie 3 (mov 824K)
Dynamics of filopodia in a hippocampal neuron. Images were acquired at 2 s/frame and are played back at 10 frames/s. Time stamp in minutes and seconds. Supplementary Movie 4 (mov 4M)
Cortical actin dynamics in the cell body of a hippocampal neuron. Images were acquired at 2 s/frame and are played back at 10 frames/s. Time stamp in minutes and seconds. Supplementary Movie 5 (mov 240K)
Cytokinetic ring formation during mitosis of MDCK cells. Images were acquired at 2 min/frame and are played back with 10 frames/s. Time stamp in hours and minutes. Supplementary Movie 6 (mov 2M)
Spreading of a primary human neutrophil on a cover petri dish coated with immune complexes. Images were acquired at 1 s/frame and are played back at 10 frames/s. Time stamp in minutes and seconds.
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