Nature Methods
- 5, 601 - 603 (2008)
Published online: 15 June 2008; | doi:10.1038/nmeth.1225
Transgenesis via permanent integration of genes in repopulating spermatogonial cells in vivo
Suveera Dhup & Subeer S Majumdar
Division of Embryo Biotechnology, National Institute of Immunology, Aruna Asaf Ali Road, New Delhi 110 067, India.
Correspondence should be addressed to Subeer S Majumdar subeer@nii.res.in
Current techniques for making transgenic mice are cumbersome, requiring trained personnel, costly infrastructure and collection of many zygotes from mice that are then killed. We developed a reproducible nonterminal technique for transfecting genes in undifferentiated spermatogonia through in vivo electroporation of the testis; about 94% of male mice electroporated with different transgenes successfully sired transgenic pups. Such electroporated males provide a valuable resource for continuous production of transgenic founders for more than a year.
MORE ARTICLES LIKE THIS
These links to content published by NPG are automatically generated.
