Nature Methods
- 5, 605 - 607 (2008)
Published online: 8 June 2008; | doi:10.1038/nmeth.1220
Lifeact: a versatile marker to visualize F-actinJulia Riedl1, 7, Alvaro H Crevenna1, 7, Kai Kessenbrock2, Jerry Haochen Yu1, Dorothee Neukirchen3, Michal Bista4, Frank Bradke3, Dieter Jenne2, Tad A Holak4, Zena Werb5, Michael Sixt6 & Roland Wedlich-Soldner11
Max Planck Institute of Biochemistry, Independent Junior Research Group Cellular Dynamics and Cell Patterning, Am Klopferspitz 18, 82152 Martinsried, Germany. 2
Max Planck Institute of Neurobiology, Department of Neuroimmunology, Am Klopferspitz 18, 82152 Martinsried, Germany. 3
Max Planck Institute of Neurobiology, Independent Junior Research Group Axonal Growth and Regeneration, Am Klopferspitz 18, 82152 Martinsried, Germany. 4
Max Planck Institute of Biochemistry, Department of Structural Cell Biology, Am Klopferspitz 18, 82152 Martinsried, Germany. 5
Department of Anatomy and The Biomedical Sciences Program, University of California, San Francisco, 513 Parnassus Avenue, San Francisco, California 94143, USA. 6
Max Planck Institute of Biochemistry, Department of Molecular Medicine, Am Klopferspitz 18, 82152 Martinsried, Germany. 7
These authors contributed equally to this work.
Correspondence should be addressed to Michael Sixt sixt@biochem.mpg.de or Roland Wedlich-Soldner wedlich@biochem.mpg.de Live imaging of the actin cytoskeleton is crucial for the study of many fundamental biological processes, but current approaches to visualize actin have several limitations. Here we describe Lifeact, a 17-amino-acid peptide, which stained filamentous actin (F-actin) structures in eukaryotic cells and tissues. Lifeact did not interfere with actin dynamics in vitro and in vivo and in its chemically modified peptide form allowed visualization of actin dynamics in nontransfectable cells.
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