Nature Methods
- 5, 609 - 612 (2008)
Published online: 30 May 2008; | doi:10.1038/nmeth.1219
In vitro whole-organ imaging: 4D quantification of growing mouse limb budsMarit J Boot1, C Henrik Westerberg2, Juanjo Sanz-Ezquerro3, James Cotterell1, 2, Ronen Schweitzer4, Miguel Torres3 & James Sharpe1, 2, 51
Medical Research Council (MRC) Human Genetics Unit, Crewe Road South, Edinburgh EH4 2XU, UK. 2
European Molecular Biology Laboratory–Centre for Genomic Regulation (EMBL-CRG) Systems Biology Unit, Universitat Pompeu Fabra, Dr. Aiguader 88, Barcelona 08003, Spain. 3
Centro Nacional de Investigaciones Cardiovasculares, Melchor Fernández Almagro, 3, Madrid 28029, Spain. 4
Shriners Hospital, Research, 3101 SW Sam Jackson Park Road, Portland, Oregon 97239, USA. 5
Institució Catalana de Recerca i Estudis Avançats (ICREA), Passeig Lluis Companys 23, Barcelona 08010, Spain.
Correspondence should be addressed to James Sharpe james.sharpe@crg.es Quantitative mapping of the normal tissue dynamics of an entire developing mammalian organ has not been achieved so far but is essential to understand developmental processes and to provide quantitative data for computational modeling. We developed a four-dimensional (4D) imaging technique that can be used to quantitatively image tissue movements and dynamic GFP expression domains in a growing transgenic mouse limb by time-lapse optical projection tomography (OPT).
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