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Nature Methods 5, 472–473 (1 June 2008) | doi:10.1038/nmeth0608-472

Turning fluorescent proteins into energy-saving light bulbs

Gert-Jan Kremers & David W Piston

The key properties of fluorescent probes include (i) wavelength of absorption and fluorescence (color), (ii) brightness and (iii) photostability. The established strategies for optimizing fluorescent proteins have primarily focused on color and brightness.