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Brief Communication

Nature Methods 5, 531–533 (1 June 2008) | doi:10.1038/nmeth.1203

Femtosecond laser nanoaxotomy lab-on-a-chip for in vivo nerve regeneration studies

Samuel X Guo , Frederic Bourgeois , Trushal Chokshi , Nicholas J Durr , Massimo A Hilliard , Nikos Chronis & Adela Ben-Yakar

A thorough understanding of nerve regeneration in Caenorhabditis elegans requires performing femtosecond laser nanoaxotomy while minimally affecting the worm. We present a microfluidic device that fulfills such criteria and can easily be automated to enable high-throughput genetic and pharmacological screenings.