Nature Methods
- 5, 475 - 489 (2008)
Published online: 29 May 2008; | doi:10.1038/nmeth.1215
Do-it-yourself guide: how to use the modern single-molecule toolkitNils G Walter, Cheng-Yen Huang, Anthony J Manzo & Mohamed A Sobhy
Department of Chemistry, Single Molecule Analysis Group, University of Michigan, 930 North University Avenue, Ann Arbor, Michigan 48109, USA.
Correspondence should be addressed to Nils G Walter nwalter@umich.edu Single-molecule microscopy has evolved into the ultimate-sensitivity toolkit to study systems from small molecules to living cells, with the prospect of revolutionizing the modern biosciences. Here we survey the current state of the art in single-molecule tools including fluorescence spectroscopy, tethered particle microscopy, optical and magnetic tweezers, and atomic force microscopy. We also provide guidelines for choosing the right approach from the available single-molecule toolkit for applications as diverse as structural biology, enzymology, nanotechnology and systems biology.
MORE ARTICLES LIKE THIS These links to content published by NPG are automatically generated.
|
