Nature Methods
- 5, 535 - 538 (2008)
Published online: 11 May 2008; | doi:10.1038/nmeth.1210
Next-generation high-density self-assembling functional protein arraysNiroshan Ramachandran, Jacob V Raphael, Eugenie Hainsworth, Gokhan Demirkan, Manuel G Fuentes, Andreas Rolfs, Yanhui Hu & Joshua LaBaer
Harvard Institute of Proteomics, Harvard Medical School, 320 Charles Street, Cambridge, Massachusetts 02141, USA.
Correspondence should be addressed to Joshua LaBaer joshua_labaer@hms.harvard.edu We developed a high-density self-assembling protein microarray, based on the nucleic acid programmable protein array (NAPPA) concept, to display thousands of proteins that are produced and captured in situ from immobilized cDNA templates. We arrayed up to 1,000 unique human cDNAs and obtained high yields of protein expression and capture with minimal variation and good reproducibility. This method will enable various experimental approaches to study protein function in high throughput.
MORE ARTICLES LIKE THIS These links to content published by NPG are automatically generated.
|
