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Nature Methods
ISSUE
May 2008, Volume 5 No 5
In This Issue
Editorial
Correspondence
Research Highlights
News and Views
Brief Communications
Articles
Technology Feature
Addendum
Application Notes
About the cover
In This Issue Top
PDF
Editorial Top
Talking points pp371 - 372
doi:10.1038/nmeth0508-371
Abstract | Full text | PDF (60K)
Correspondence Top
Two-photon dual-color imaging using fluorescent proteins pp373 - 374
Hiroyuki Kawano, Takako Kogure, Yukiko Abe, Hideaki Mizuno & Atsushi Miyawaki
doi:10.1038/nmeth0508-373
Full text | PDF (327K)  | Supplementary Information
Unexpected failure rates for modular assembly of engineered zinc fingers pp374 - 375
Cherie L Ramirez, Jonathan E Foley, David A Wright, Felix Müller-Lerch, Shamim H Rahman, Tatjana I Cornu, Ronnie J Winfrey, Jeffry D Sander, Fengli Fu, Jeffrey A Townsend, Toni Cathomen, Daniel F Voytas & J Keith Joung
doi:10.1038/nmeth0508-374
Full text | PDF (108K)  | Supplementary Information
ADVERTISEMENT
Research Highlights Top
Two pictures are better than one p377
Daniel Evanko
doi:10.1038/nmeth0508-377
Abstract | Full text | PDF (122K)
Protein structure determination... blindfolded pp378 - 379
Allison Doerr
doi:10.1038/nmeth0508-378a
Abstract | Full text | PDF (187K)
Knockouts with a touch of a zinc finger pp378 - 379
Irene Kaganman
doi:10.1038/nmeth0508-378b
Abstract | Full text | PDF (187K)
News in brief p379
doi:10.1038/nmeth0508-379
Full text | PDF (84K)
Follow the ubiquitin p380
Katherine Stevens
doi:10.1038/nmeth0508-380
Abstract | Full text | PDF (83K)
News and Views Top
BAC to the future: functional genomics in mammals pp383 - 384
Assen Roguev & Nevan J Krogan
doi:10.1038/nmeth0508-383
Abstract | Full text | PDF (246K)
See also: Article by Poser et al.
Metastasis: two assays explore the two roads traveled pp384 - 385
Diane Renee Bielenberg
doi:10.1038/nmeth0508-384
Abstract | Full text | PDF (1,544K)
See also: Article by Bruyère et al.  | Article by Alajati et al.
Super-resolution light microscopy goes live pp385 - 387
Mats G L Gustafsson
doi:10.1038/nmeth0508-385
Abstract | Full text | PDF (1,654K)
See also: Article by Shroff et al.
Brief Communications Top
Improved genetic manipulation of human embryonic stem cells pp389 - 392
Stefan R Braam, Chris Denning, Stieneke van den Brink, Peter Kats, Ron Hochstenbach, Robert Passier & Christine L Mummery
Published online: 06 April 2008 | doi:10.1038/nmeth.1200
Abstract | Full text | PDF (435K)  | Supplementary Information
Suspended-drop electroporation for high-throughput delivery of biomolecules into cells pp393 - 395
Emmanuel G Guignet & Tobias Meyer
Published online: 13 April 2008 | doi:10.1038/nmeth.1201
Abstract | Full text | PDF (317K)  | Supplementary Information
Monovalent, reduced-size quantum dots for imaging receptors on living cells pp397 - 399
Mark Howarth, Wenhao Liu, Sujiet Puthenveetil, Yi Zheng, Lisa F Marshall, Michael M Schmidt, K Dane Wittrup, Moungi G Bawendi & Alice Y Ting
Published online: 20 April 2008 | doi:10.1038/nmeth.1206
Abstract | Full text | PDF (243K)  | Supplementary Information
Fluorescent protein FRET pairs for ratiometric imaging of dual biosensors pp401 - 403
Hui-wang Ai, Kristin L Hazelwood, Michael W Davidson & Robert E Campbell
Published online: 20 April 2008 | doi:10.1038/nmeth.1207
Abstract | Full text | PDF (211K)  | Supplementary Information
Straightforward ladder sequencing of peptides using a Lys-N metalloendopeptidase pp405 - 407
Nadia Taouatas, Madalina M Drugan, Albert J R Heck & Shabaz Mohammed
Published online: 20 April 2008 | doi:10.1038/nmeth.1204
Abstract | Full text | PDF (143K)  | Supplementary Information
Articles Top
BAC TransgeneOmics: a high-throughput method for exploration of protein function in mammals pp409 - 415
Ina Poser, Mihail Sarov, James R A Hutchins, Jean-Karim Hériché, Yusuke Toyoda, Andrei Pozniakovsky, Daniela Weigl, Anja Nitzsche, Björn Hegemann, Alexander W Bird, Laurence Pelletier, Ralf Kittler, Sujun Hua, Ronald Naumann, Martina Augsburg, Martina M Sykora, Helmut Hofemeister, Youming Zhang, Kim Nasmyth, Kevin P White, Steffen Dietzel, Karl Mechtler, Richard Durbin, A Francis Stewart, Jan-Michael Peters, Frank Buchholz & Anthony A Hyman
Published online: 06 April 2008 | doi:10.1038/nmeth.1199
Abstract | Full text | PDF (832K)  | Supplementary Information
See also: News and Views by Roguev & Krogan
Live-cell photoactivated localization microscopy of nanoscale adhesion dynamics pp417 - 423
Hari Shroff, Catherine G Galbraith, James A Galbraith & Eric Betzig
Published online: 13 April 2008 | doi:10.1038/nmeth.1202
Abstract | Full text | PDF (813K)  | Supplementary Information
See also: News and Views by Gustafsson
A metric for odorant comparison pp425 - 429
Rafi Haddad, Rehan Khan, Yuji K Takahashi, Kensaku Mori, David Harel & Noam Sobel
Published online: 30 March 2008 | doi:10.1038/nmeth.1197
Abstract | Full text | PDF (169K)  | Supplementary Information
Modeling lymphangiogenesis in a three-dimensional culture system pp431 - 437
Françoise Bruyère, Laurence Melen-Lamalle, Silvia Blacher, Guy Roland, Marc Thiry, Lieve Moons, Francis Frankenne, Peter Carmeliet, Kari Alitalo, Claude Libert, Jonathan P Sleeman, Jean-Michel Foidart & Agnès Noël
Published online: 20 April 2008 | doi:10.1038/nmeth.1205
Abstract | Full text | PDF (526K)  | Supplementary Information
See also: News and Views by Bielenberg
Spheroid-based engineering of a human vasculature in mice pp439 - 445
Abdullah Alajati, Anna M Laib, Holger Weber, Anja M Boos, Arne Bartol, Kristian Ikenberg, Thomas Korff, Hanswalter Zentgraf, Cynthia Obodozie, Ralph Graeser, Sven Christian, Günter Finkenzeller, G Björn Stark, Mélanie Héroult & Hellmut G Augustin
Published online: 06 April 2008 | doi:10.1038/nmeth.1198
Abstract | Full text | PDF (523K)  | Supplementary Information
See also: News and Views by Bielenberg
Technology Feature Top
SNP genotyping: six technologies that keyed a revolution pp447 - 453
Jeffrey Perkel & Nathan Blow
doi:10.1038/nmeth0508-447
Abstract | Full text | PDF (551K)
Addendum Top
Addendum: Highly inclined thin illumination enables clear single-molecule imaging in cells p455
doi:10.1038/nmeth0508-455
Full text | PDF (49K)
Application Notes Top
3K Long-Tag Paired End sequencing with the Genome Sequencer FLX System 
Thomas Jarvie & Timothy Harkins

Abstract | Full text | PDF (329K)
High-content analysis with AxioVision ASSAYbuilder™ 
Horst Wolff

Abstract | Full text | PDF (487K)
  Top

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ISSN: 1548-7091
EISSN: 1548-7105
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