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Nature Methods 5, 374–375 (1 May 2008) | doi:10.1038/nmeth0508-374

Unexpected failure rates for modular assembly of engineered zinc fingers

Cherie L Ramirez , Jonathan E Foley , David A Wright , Felix M|[uuml]|ller-Lerch , Shamim H Rahman , Tatjana I Cornu , Ronnie J Winfrey , Jeffry D Sander , Fengli Fu , Jeffrey A Townsend , Toni Cathomen , Daniel F Voytas & J Keith Joung

To the editor: Zinc-finger nucleases (ZFNs) consist of an engineered zinc-finger array fused to a nuclease domain. Dimers of ZFNs can create targeted double-strand DNA breaks, which can stimulate highly efficient gene targeting in many cell types (Supplementary Fig.