Nature Methods
- 5, 311 - 313 (2008)
Published online: 16 March 2008; | doi:10.1038/nmeth.1196
In vivo immunogold labeling confirms large-scale chromatin folding motifsIgor Kireev, Margot Lakonishok, Wenqiu Liu, Vishwas N Joshi, Rick Powell & Andrew S Belmont Supplementary Text and Figures (pdf 2M)
Supplementary Figures 1–4, Supplementary Methods Supplementary Movie 1 (mov 2M)
Tilted projections showing gene amplified region from PDC cell labeled in vivo with anti-GFP Nanogold antibodies. Large-scale chromatin fibers of 120-170 nm diameter can be visualized corresponding to the GFP-lac repressor staining of the lac operator containing, amplified transgene array. Projection step size 5 degrees. Scale bar, 500 nm. Supplementary Movie 2 (mov 1M)
Rocking projections of a mid-S phase, DNA replication focus, as identified by GFP-PCNA labeled in vivo with anti-GFP Nanogold antibodies. Note the appearance of a uniform distribution of label through the replication focus. Projection step size 5 degrees. Cell was permeabilized prior to fixation to reveal surrounding chromatin (darkly stained material).
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