Nature Methods
- 5, 299 - 302 (2008)
Published online: 9 March 2008; | doi:10.1038/nmeth.1190
Genetic control of neuronal activity in mice conditionally expressing TRPV1Benjamin R Arenkiel, Marguerita E Klein, Ian G Davison, Lawrence C Katz & Michael D Ehlers
Howard Hughes Medical Institute, Department of Neurobiology, Duke University Medical Center, Box 3209, Durham, North Carolina 27710, USA.
Correspondence should be addressed to Michael D Ehlers ehlers@neuro.duke.edu Here we describe a knock-in mouse model for Cre-loxP–based conditional expression of TRPV1 in central nervous system neurons. Expression of Cre recombinase using biolistics, lentivirus or genetic intercrosses triggered heterologous expression of TRPV1 in a cell-specific manner. Application of the TRPV1 ligand capsaicin induced strong inward currents, triggered action potentials and activated stereotyped behaviors, allowing cell type–specific chemical genetic control of neuronal activity in vitro and in vivo.
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